A simplified culture system to examine soluble factor interactions between mammalian cells

Chem Commun (Camb). 2014 May 25;50(40):5279-81. doi: 10.1039/c3cc47507g. Epub 2013 Nov 14.

Abstract

Interactions between different cell types play critical roles in normal development and disease, but remain challenging to analyse. Here, a co-culture system is described that overcomes many of the limitations of existing methods, is simple to construct and modify, and is compatible with standard cellular and molecular assays.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Adhesion
  • Cells, Cultured
  • Coculture Techniques
  • Extracellular Signal-Regulated MAP Kinases / metabolism*
  • Female
  • Gelatin / metabolism*
  • Humans
  • Monocytes / cytology
  • Monocytes / metabolism*
  • Ovarian Neoplasms / metabolism*
  • Ovarian Neoplasms / pathology
  • Phosphorylation

Substances

  • Gelatin
  • Extracellular Signal-Regulated MAP Kinases