MALDI-TOF MS for identification of porcine Brachyspira species

Lett Appl Microbiol. 2014 Mar;58(3):292-8. doi: 10.1111/lam.12189. Epub 2013 Nov 18.

Abstract

The aim of this study consisted in evaluating MALDI-TOF MS as a tool for the identification of the genus Brachyspira (B.) and its relevant species for the pig industry. First, a database was created with 30 control strains, and superspectra for five different porcine Brachyspira species were calculated. In a second step, 67 field isolates were investigated using MALDI-TOF MS, and results were compared to those obtained using nox gene-based RFLP (reference method) and biochemical tests. Among the 67 field isolates, five different Brachyspira species were detected using nox gene-based RFLP analysis. MALDI-TOF MS analysis correctly assigned all isolates to the genus Brachyspira and identified all isolates from B. hyodysenteriae (29/29), B. pilosicoli (11/11), B. intermedia (4/4) and B. innocens (11/11). In terms of B. murdochii, MALDI-TOF MS assigned one of 12 isolates ambiguously as B. innocens/B. murdochii. The results of this study indicate that MALDI-TOF MS facilitates the diagnosis of swine dysentery and porcine intestinal spirochaetosis.

Significance and impact of the study: Current methods for the discrimination of pathogenic Brachyspira hyodysenteriae and Brachyspira pilosicoli from Brachyspira species with low pathogenic potential have proven to be laborious and time-consuming and are therefore not suitable for routine diagnostics. This study describes the evaluation of MALDI-TOF MS for the identification of different porcine Brachyspira species in routine diagnostic laboratories. The results suggest that MALDI-TOF MS is an effective method for the identification of porcine Brachyspira spp. and accelerates diagnosis of swine dysentery and porcine intestinal spirochaetosis.

Keywords: Brachyspira; MALDI-TOF MS; pigs; porcine intestinal spirochaetosis; swine dysentery.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Typing Techniques / methods*
  • Base Sequence
  • Brachyspira / chemistry*
  • Brachyspira / classification
  • Brachyspira / genetics
  • Brachyspira / isolation & purification*
  • Gram-Negative Bacterial Infections / diagnosis
  • Gram-Negative Bacterial Infections / microbiology
  • Gram-Negative Bacterial Infections / veterinary*
  • Molecular Sequence Data
  • Phylogeny
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Sus scrofa
  • Swine
  • Swine Diseases / diagnosis
  • Swine Diseases / microbiology*

Associated data

  • GENBANK/KF680560
  • GENBANK/KF680561
  • GENBANK/KF680562
  • GENBANK/KF680563
  • GENBANK/KF680564
  • GENBANK/KF680565
  • GENBANK/KF680566
  • GENBANK/KF680567
  • GENBANK/KF680568
  • GENBANK/KF680569
  • GENBANK/KF680570
  • GENBANK/KF680571
  • GENBANK/KF680572
  • GENBANK/KF680573
  • GENBANK/KF680574
  • GENBANK/KF680575
  • GENBANK/KF680576
  • GENBANK/KF680577
  • GENBANK/KF680578
  • GENBANK/KF680579
  • GENBANK/KF680580
  • GENBANK/KF680581