Cloning part of the region encoding biosynthetic enzymes for surface antigen (O-antigen) of Salmonella typhimurium

Mol Gen Genet. 1986 Apr;203(1):172-6. doi: 10.1007/BF00330399.

Abstract

The rfb gene cluster of Salmonella typhimurium encodes the enzymes required for the biosynthesis of the O-Antigen. A part of it has been cloned in plasmid vectors pBR322 and pUC9 using an adjacent, previously cloned, part of the his operon (Barnes 1981) as a molecular probe for the first clone. A detailed restriction enzyme map of 7.57 kb of rfb DNA is presented and the approximate locations of two of the genes, rfbK and rfbM have been defined.

MeSH terms

  • Antigens, Bacterial / genetics*
  • Antigens, Surface / genetics*
  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • Genes*
  • Genes, Bacterial*
  • Genetic Vectors
  • Lipopolysaccharides / genetics*
  • O Antigens
  • Plasmids
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / immunology

Substances

  • Antigens, Bacterial
  • Antigens, Surface
  • Lipopolysaccharides
  • O Antigens
  • DNA Restriction Enzymes