Auto-attraction of Neural Precursors and Their Neuronal Progeny Impairs Neuronal Migration

Nat Neurosci. 2014 Jan;17(1):24-6. doi: 10.1038/nn.3583. Epub 2013 Nov 17.

Abstract

Limited neuronal migration into host brain tissue is a key challenge in neural transplantation. We found that one important mechanism underlying this phenomenon is an intrinsic chemotactic interaction between the grafted neural precursor cells (NPCs) and their neuronal progeny. NPCs secrete the receptor tyrosine kinase ligands FGF2 and VEGF, which act as chemoattractants for neurons. Interference with these signaling pathways resulted in enhanced migration of human neurons from neural clusters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / pharmacology
  • Cell Differentiation
  • Cell Movement / physiology*
  • Cells, Cultured
  • Diffusion Chambers, Culture
  • Embryonic Stem Cells / physiology*
  • Enzyme Inhibitors / pharmacology
  • Female
  • Fibroblast Growth Factor 2 / immunology
  • Fibroblast Growth Factor 2 / pharmacology
  • Flow Cytometry
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Hippocampus / cytology
  • Humans
  • Mice
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism
  • Nestin / metabolism
  • Neural Stem Cells / physiology*
  • Neurons / physiology*
  • Neuropeptides / genetics
  • Neuropeptides / metabolism
  • Organ Culture Techniques
  • Vascular Endothelial Growth Factor A / immunology
  • Vascular Endothelial Growth Factor A / pharmacology

Substances

  • Antibodies
  • Enzyme Inhibitors
  • Microtubule-Associated Proteins
  • Nestin
  • Neuropeptides
  • Vascular Endothelial Growth Factor A
  • doublecortin protein
  • Fibroblast Growth Factor 2
  • Green Fluorescent Proteins