Detection of femtomole and sub-femtomole levels of peptides by tandem magnetic sector/reflectron time-of-flight mass spectrometry and matrix-assisted laser desorption ionization

F H Strobel; T Solouki; M A White; D H Russell

doi:10.1016/1044-0305(91)80066-G

Detection of femtomole and sub-femtomole levels of peptides by tandem magnetic sector/reflectron time-of-flight mass spectrometry and matrix-assisted laser desorption ionization

J Am Soc Mass Spectrom. 1991 Jan;2(1):91-4. doi: 10.1016/1044-0305(91)80066-G.

Authors

F H Strobel¹, T Solouki, M A White, D H Russell

Affiliation

¹ Department of Chemistry, Texas A & M University, 77843, College Station, TX, USA.

PMID: 24242094
DOI: 10.1016/1044-0305(91)80066-G

Abstract

This article describes results of low-level (sub-femtomole) detection of peptides by matrix-assisted laser desorption ionization. The matrix-assisted laser desorption ionization method can be used for low-level detection of the parent ion, either [M + H](+) or [M + Na](+), and collision-induced dissociation of the parent ion can be performed at the picomole level. The instrument used for these studies is a novel high-performance magnetic sector (electric(E)/magnetic(B) sector)/reflectron time-of-flight (TOP) tandem mass spectrometer (EB/TOF).