Adoptive immunotherapy: New assay for the identification of T cells with optimal avidity

Oncoimmunology. 2013 Oct 1;2(10):e26199. doi: 10.4161/onci.26199. Epub 2013 Sep 12.


T cells expressing high avidity T-cell receptors (TCRs) have been shown to mediate superior therapeutic effects. A novel koff-rate assay allows for the quantitative and reproducible assessment of the avidity of TCRs for their ligands directly on living T cells, ex vivo. This assay might facilitate the selection of T cells with an optimal avidity for their target, hence favoring the development of adoptive immunotherapeutic regimens.

Keywords: CD8+ cytotoxic T cell; Koff rate; T cell; T cell receptor; avidity.