Chemopreventive and therapeutic activity of dietary blueberry against estrogen-mediated breast cancer

J Agric Food Chem. 2014 May 7;62(18):3963-71. doi: 10.1021/jf403734j. Epub 2014 Apr 15.


Berries are gaining increasing importance lately for their chemopreventive and therapeutic potential against several cancers. In earlier studies, a blueberry-supplemented diet has shown protection against 17β-estradiol (E2)-mediated mammary tumorigenesis. This study tested both preventive and therapeutic activities of diet supplemented with whole blueberry powder (50:50 blend of Tifblue and Rubel). Animals received 5% blueberry diet, either 2 weeks prior to or 12 weeks after E2 treatment in preventive and therapeutic groups, respectively. Both interventions delayed the tumor latency for palpable mammary tumors by 28 and 37 days, respectively. Tumor volume and multiplicity were also reduced significantly in both modes. The effect on mammary tumorigenesis was largely due to down-regulation of CYP 1A1 and ER-α gene expression and also favorable modulation of microRNA (miR-18a and miR-34c) levels. These data suggest that the blueberry blend tested is effective in inhibiting E2-mediated mammary tumorigenesis in both preventive and therapeutic modes.

Keywords: ACI rats; blood chemistry; blueberry; breast cancer; chemoprevention; chemotherapy; estrogen; hematopoietic parameters; miRNA modulation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blueberry Plants / metabolism*
  • Breast Neoplasms / diet therapy*
  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / prevention & control
  • Cytochrome P-450 CYP1A1 / genetics
  • Cytochrome P-450 CYP1A1 / metabolism
  • Estradiol / metabolism*
  • Estrogen Receptor alpha / genetics
  • Estrogen Receptor alpha / metabolism
  • Estrogens / metabolism*
  • Female
  • Fruit / metabolism*
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • Rats
  • Rats, Inbred ACI


  • Estrogen Receptor alpha
  • Estrogens
  • MicroRNAs
  • Estradiol
  • Cytochrome P-450 CYP1A1