Abstract
Bioassay-guided fractionation of the EtOAc extract from Disporum viridescens roots led to the isolation of five new benzyl benzoate glycosides, BBGs (1-5). The neuroprotective activities of the BBGs were screened using neuronal HT22 hippocampal cells. BBG-D (4) significantly protected murine hippocampal HT22 cells against glutamate-induced neurotoxicity by maintaining the antioxidative defense systems such as superoxide dismutase, glutathione reductase, glutathione peroxidase, and the glutathione content. BBG-D, in a dose-and time-dependent manner, increased HO-1 expression through the selective activation of pERK signaling among the MAPK pathways. These results suggest that BBG-D could be a promising candidate for the treatment of neurodegenerative diseases related to glutamate-induced oxidative neuronal cytotoxicity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / metabolism
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Antioxidants / pharmacology
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Benzoates / chemistry
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Benzoates / pharmacology
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Cell Survival / drug effects
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Glutamic Acid / metabolism
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Glutamic Acid / pharmacology*
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Glycosides / chemistry
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Glycosides / isolation & purification*
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Glycosides / pharmacology*
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Heme Oxygenase-1 / metabolism
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Hippocampus / cytology
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Hippocampus / metabolism
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Liliaceae / chemistry*
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Mice
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Molecular Structure
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Neurons / metabolism
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Neuroprotective Agents / chemistry
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Neuroprotective Agents / isolation & purification*
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Neuroprotective Agents / pharmacology*
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Oxidative Stress / drug effects
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Plant Roots / chemistry
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Superoxide Dismutase / drug effects
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Superoxide Dismutase / metabolism
Substances
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Antioxidants
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Benzoates
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Glycosides
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Neuroprotective Agents
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Glutamic Acid
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Heme Oxygenase-1
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Superoxide Dismutase
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benzyl benzoate