C1q binding to dengue virus decreases levels of infection and inflammatory molecules transcription in THP-1 cells

Virus Res. 2014 Jan 22;179:231-4. doi: 10.1016/j.virusres.2013.11.007. Epub 2013 Nov 15.

Abstract

Dengue virus infection elicits a spectrum of clinical presentations ranging from asymptomatic to severe disease. The mechanisms leading to severe dengue are not known, however it has been reported that the complement system is hyper-activated in severe dengue. Screening of complement proteins demonstrated that C1q, a pattern recognition molecule, can bind directly to dengue virus envelope protein and to whole dengue virus serotype 2. Incubation of dengue virus serotype 2 with C1q prior to infection of THP-1 cells led to decreased virus infectivity and modulation of mRNA expression of immunoregulatory molecules suggesting reduced inflammatory responses.

Keywords: C1q; Classical complement pathway; Dengue; Dengue fever; Dengue hemorrhagic fever; Viral persistence.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • B7-2 Antigen / genetics
  • B7-2 Antigen / immunology
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / immunology
  • Cell Line
  • Complement C1q / immunology
  • Complement C1q / metabolism*
  • Dengue / genetics*
  • Dengue / immunology
  • Dengue / metabolism
  • Dengue / virology
  • Dengue Virus / classification
  • Dengue Virus / immunology
  • Dengue Virus / physiology*
  • Humans
  • Inflammation Mediators / immunology*
  • Lectins, C-Type / genetics
  • Lectins, C-Type / immunology
  • Lipopolysaccharide Receptors / genetics
  • Lipopolysaccharide Receptors / immunology
  • Protein Binding
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / immunology
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / metabolism

Substances

  • B7-2 Antigen
  • Cell Adhesion Molecules
  • DC-specific ICAM-3 grabbing nonintegrin
  • Inflammation Mediators
  • Lectins, C-Type
  • Lipopolysaccharide Receptors
  • Receptors, Cell Surface
  • Viral Envelope Proteins
  • Complement C1q