Effect of near-infrared light exposure on mitochondrial signaling in C2C12 muscle cells

Mitochondrion. 2014 Jan;14(1):42-8. doi: 10.1016/j.mito.2013.11.001. Epub 2013 Nov 15.


Near-infrared (NIR) light is a complementary therapy used to treat musculoskeletal injuries but the underlying mechanisms are unclear. Acute NIR light treatment (~800-950 nm; 22.8 J/cm(2)) induced a dose-dependent increase in mitochondrial signaling (AMPK, p38 MAPK) in differentiated muscle cells. Repeated NIR light exposure (4 days) appeared to elevate oxidative stress and increase the upstream mitochondrial regulatory proteins AMPK (3.1-fold), p38 (2.8-fold), PGC-1α (19.7%), Sirt1 (26.8%), and reduced RIP140 (23.2%), but downstream mitochondrial regulation/content (Tfam, NRF-1, Sirt3, cytochrome c, ETC subunits) was unaltered. Our data indicates that NIR light alters mitochondrial biogenesis signaling and may represent a mechanistic link to the clinical benefits.

Keywords: Mitochondrial biogenesis; NIR light; Oxidative stress; Photobiomodulation; Reactive oxygen species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Infrared Rays*
  • Mice
  • Mitochondria / physiology*
  • Mitochondria / radiation effects*
  • Mitochondrial Turnover / radiation effects*
  • Muscle Cells / physiology*
  • Muscle Cells / radiation effects*
  • Signal Transduction*