Abstract
Neonatal rat sciatic nerve Schwann cells in monolayer culture are stimulated to proliferate and to express a lipid and a protein characteristic of myelin by agents which raise intracellular cyclic adenosine 3',5'-monophosphate. Both glial growth factor and axolemmal fragments increase the rate of mitosis of cultured rat and human Schwann cells. Rat Schwann cell mitosis is enhanced by a soluble factor produced by concanavalin A-stimulated blood mononuclear cells and inhibited by lead salts. Schwann-like cells cultured from human dermal and plexiform neurofibromas resemble normal human Schwann cells in phenotype and response to mitogens.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
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Review
MeSH terms
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Animals
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Animals, Newborn
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Axons / physiology
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Cell Differentiation* / drug effects
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Cell Division* / drug effects
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Cell Separation
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Cells, Cultured
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Colforsin / pharmacology
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Culture Media
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Culture Techniques
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Cyclic AMP / analogs & derivatives
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Fibronectins / pharmacology
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Glia Maturation Factor
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Humans
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Laminin / pharmacology
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Lead / pharmacology
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Lymphokines / pharmacology
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Myelin Basic Protein / metabolism
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Nerve Tissue Proteins / pharmacology
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Neurofibromatosis 1 / pathology
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Rats
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Schwann Cells / cytology*
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Schwann Cells / drug effects
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Sciatic Nerve / cytology
Substances
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Culture Media
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Fibronectins
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Glia Maturation Factor
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Laminin
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Lymphokines
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Myelin Basic Protein
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Nerve Tissue Proteins
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Colforsin
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Lead
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Cyclic AMP