Applications of Lactobacillus rhamnosus spent culture supernatant in cosmetic antioxidation, whitening and moisture retention applications

Molecules. 2013 Nov 15;18(11):14161-71. doi: 10.3390/molecules181114161.


This study was aimed at investigating the antioxidant, whitening, and moisture-retention properties of Lactobacillus rhamnosus spent culture supernatant (Lr-SCS) in cosmetic applications. Results reveal that Lr-SCS effectively and gradually scavenges 1,1-diphenyl-2-picrylhydrazyl as well as 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cations, and increases reducing power in a dose-dependent manner. Additionally, Lr-SCS can also suppress tyrosinase activity in vitro and effectively promote moisture retention. Heat treatment at 100 °C for 30 min does not influence the functions of Lr-SCS. We conclude that Lr-SCS can be used effectively in skin care cosmetics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / chemistry*
  • Biphenyl Compounds / chemistry
  • Bleaching Agents / chemistry*
  • Cosmetics / chemistry*
  • Culture Media, Conditioned / chemistry
  • Culture Media, Conditioned / pharmacology*
  • Enzyme Activation / drug effects
  • Lactobacillus rhamnosus / chemistry*
  • Monophenol Monooxygenase / metabolism
  • Oxidation-Reduction / drug effects
  • Picrates / chemistry


  • Antioxidants
  • Biphenyl Compounds
  • Bleaching Agents
  • Cosmetics
  • Culture Media, Conditioned
  • Picrates
  • 1,1-diphenyl-2-picrylhydrazyl
  • Monophenol Monooxygenase