Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annuus L.) on a high sucrose-containing medium

J J Finer

doi:10.1007/BF00269564

Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annuus L.) on a high sucrose-containing medium

Plant Cell Rep. 1987 Oct;6(5):372-4. doi: 10.1007/BF00269564.

Author

J J Finer¹

Affiliation

¹ Department of Agronomy, Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA.

PMID: 24248849
DOI: 10.1007/BF00269564

Abstract

Somatic embryos of sunflower (Helianthus annuus) were obtained by placing immature zygotic embryos on a high sucrose (12%) containing medium. The somatic embryos were first observed 6 days after culture and a callus intermediate was not formed. Histological examination revealed the classical stages of embryo development. The somatic embryos proliferated directly from the surface of the zygotic embryos and germinated after placement on a low sucrose medium. Seeds have been obtained from regenerated plants.