Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 May;164(2):207-14.
doi: 10.1007/BF00396083.

Establishment and Maintenance of Friable, Embryogenic Maize Callus and the Involvement of L-proline

Affiliations

Establishment and Maintenance of Friable, Embryogenic Maize Callus and the Involvement of L-proline

C L Armstrong et al. Planta. .

Abstract

Friable, embryogenic maize (Zea mays L.), inbred line A188, callus was established and maintained for more than one year without apparent loss of friability or embryogenic potential. Embryoid development was abundant in these cultures and plants were easily regenerated. Frequencies of friable-callus initiation and somatic-embryoid formation increased linearly with addition to N6 medium (C.C. Chu et al. 1975, Sci. Sin. [Peking] 18, 659-668) of up to 25 mM L-proline. Proline additions up to 9 mM to MS medium (inorganic elements of T. Murashige and F. Skoog 1962, Physiol. Plant. 15, 473-497, plus 0.5 mg 1(-1) thiamine hydrochloride and 150 mg 1(-1) L-asparagine monohydrate) did not stimulate embryoid formation. A major part of the difference between MS and N6 media could be attributed to their respective inorganic nitrogen components. L-Glutamine was not a satisfactory substitute for L-proline. Of 111 regenerated plants grown to maturity from three independent friable, embryogenic cell lines ranging in age from three to seven months, only four plants were abnormal based on morphology and pollen sterility. Seed was produced by 77% of the regenerated plants.

Similar articles

See all similar articles

Cited by 78 articles

See all "Cited by" articles

References

    1. Planta. 1983 Apr;157(5):385-91 - PubMed
    1. Plant Physiol. 1983 Sep;73(1):142-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Jan;79(2):559-63 - PubMed
    1. Science. 1984 Jun 8;224(4653):1064-8 - PubMed
    1. Theor Appl Genet. 1982 Jun;62(2):109-12 - PubMed

LinkOut - more resources

Feedback