Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. Winter 2013;12(Suppl):31-6.

Development of a Combined Solution Formulation of Atropine Sulfate and Obidoxime Chloride for Autoinjector and Evaluation of Its Stability

Affiliations
Free PMC article

Development of a Combined Solution Formulation of Atropine Sulfate and Obidoxime Chloride for Autoinjector and Evaluation of Its Stability

Hossein Ali Ettehadi et al. Iran J Pharm Res. .
Free PMC article

Abstract

Atropine (AT) and oximes, alone or in combination, have been proven greatly valuable therapeutics in the treatment of organophosphates intoxications. An injectable mixture of AT and obidoxime (OB) was formulated for the administration by automatic self-injector. The aqueous single dose solution contained 275 mg obidoxime chloride and 2.5 mg atropine sulfate per 1 mL (220 mg and 2 mg per 0.8 effective dose, respectively). The final solution was sterilized by filtration through a 0.22 μm pore size filter. This more concentrated solution allowed to use a smaller size and lighter weight cartridge. Quality control tests, including assay of the two major compounds were performed separately, using reversed-phase HPLC methods. Besides, the stability test was carried out according to ICH guideline for the accelerated test. The obtained results showed that the proposed formulation is stable over a period of 2 years after preparation.

Keywords: Atropine; Injectable solution; Obidoxime; Stability test.

Figures

Figure 1
Figure 1
Chemical structures of atropine sulfate (a) and obidoxime chloride (b).
Figure 2
Figure 2
A schematic diagram of the autoinjector device with the cartridge inside containing mixed solution of atropine and obidoxime.
Figure 3
Figure 3
Chromatograms showing (A) separation of AT sulfate (1), tropic acid (2), obidoxime (3) and phenol in a standard solution containing 0.05 mg/mL of each compound, and (B) separation of components of a cartridge.

Similar articles

See all similar articles

References

    1. M , Buckley NA, Eyer P and Dawson AH. Management of acute organophosphiorous pesticide poisoning. Lancet. 2008;371:597–607. - PMC - PubMed
    1. Leibson T and Lifshitz M. Organophosphate and Carbamate Poisoning: Review of the Current Literature and Summary of Clinical and Laboratory Experience in Southern Israel. IMAJ. 2008;10:767–770. - PubMed
    1. Petroiani GA, Hasan MY, Arafat K, Nurulain SM, Schmitt A. Weak inhibitors protect cholinesterases from stronger inhibitors (Dichlorvos) Int. J. Toxicol. 2005;24:79–86. - PubMed
    1. Worek F, Krichner T, Szinicz L. Effect of AT and bispyridine oxime on respiratory and circulatory finction in guinea pigs poisoned with sarin. Toxicology. 1995;95:123–33. - PubMed
    1. Thiermann H, Eyer F, Felgenhauer N, Pfab R, Zilke T, Eyer P, Worek F. Phamacokinetics of obidoxime in patients poisoned with organophosphorous compounds. Toxicology letters. 2010;197:236–242. - PubMed

LinkOut - more resources

Feedback