The mutagenic effect of gamma rays on leaf protoplasts of haploid and dihaploid Nicotiana plumbaginifolia, estimated by valine resistance mutation frequencies

E Nielsen; E Selva; C Sghirinzetti; M Devreux

doi:10.1007/BF00304909

The mutagenic effect of gamma rays on leaf protoplasts of haploid and dihaploid Nicotiana plumbaginifolia, estimated by valine resistance mutation frequencies

Theor Appl Genet. 1985 Jun;70(3):259-64. doi: 10.1007/BF00304909.

Authors

E Nielsen¹, E Selva, C Sghirinzetti, M Devreux

Affiliation

¹ Biology Group, Directorate General XII, J.R.C. of European Communities, I-21020, Ispra, Italy.

PMID: 24252919
DOI: 10.1007/BF00304909

Abstract

Leaf protoplasts isolated from haploid and dihaploid Nicotiana plumbaginifolia plantlets were treated with different doses of gamma-rays and their survival was determined by scoring for plating efficiency at each irradiation dose. A fixed number of surviving protoplast-derived colonies was then plated in the presence of inhibitory concentrations of L-valine and incubated until growing resistant calli could be scored and mutation rates calculated. Though haploid protoplasts were found to be a little more sensitive than dihaploids to the lethal effect of radiation, the two dose-response curves of gamma-rays that induced mutagenesis were very similar. The irradiation dose capable of causing a ten-fold increase of spontaneous mutation frequencies was about 500 rads with both haploid and dihaploid protoplasts.