Late enzymes of vindoline biosynthesis : S-Adenosyl-L-methionine: 11-O-demethyl-17-O-deacetylvindoline 11-O-methyltransferase and unspecific acetylesterase
- PMID: 24254077
- DOI: 10.1007/BF00269893
Late enzymes of vindoline biosynthesis : S-Adenosyl-L-methionine: 11-O-demethyl-17-O-deacetylvindoline 11-O-methyltransferase and unspecific acetylesterase
Abstract
From differentiated plants of Catharanthus roseus (L.) G. Don we have isolated a specific enzyme of the vindoline biosynthetic pathway catalysing the S-adenosylmethionine-dependent methylation of 11-O-demethyl-17-O-deacetyl-vindoline. The enzyme we named S-adenosyl-L-methionine : 11-O-demethyl-17-O-deacetylvindoline 11-O-methyltransferase. This transferase exhibits a high substrate specificity. Obviously the O-methylation at C-11 precedes the O-acetylation at the C-17 position during the biosynthesis of vindoline.A second enzyme was detected which hydrolyses the acetyl function of vindoline. The distribution of this acetylesterase in C. roseus plants demonstrates that the enzyme is not specifically associated with the vindoline distribution in the plant material. Most probably this enzyme plays no essential role in the biosynthesis of vindoline.
Similar articles
-
Late enzymes of vindoline biosynthesis. Acetyl-CoA: 17-O-deacetylvindoline 17-O-acetyl-transferase.Plant Cell Rep. 1985 Dec;4(6):333-6. doi: 10.1007/BF00269892. Plant Cell Rep. 1985. PMID: 24254076
-
Subcellular Localization of Enzymes Involved in Indole Alkaloid Biosynthesis in Catharanthus roseus.Plant Physiol. 1987 Dec;85(4):1099-102. doi: 10.1104/pp.85.4.1099. Plant Physiol. 1987. PMID: 16665811 Free PMC article.
-
Purification of acetyl-CoA: 17-O-deacetylvindoline 17-O-acetyltransferase from Catharanthus roseus leaves.Plant Cell Rep. 1990 Mar;8(10):613-6. doi: 10.1007/BF00270066. Plant Cell Rep. 1990. PMID: 24232684
-
Spatial organization of the vindoline biosynthetic pathway in Catharanthus roseus.J Plant Physiol. 2011 Apr 15;168(6):549-57. doi: 10.1016/j.jplph.2010.08.018. Epub 2010 Nov 2. J Plant Physiol. 2011. PMID: 21047699
-
[Advance in biosynthesis of terpenoid indole alkaloids and its regulation in Catharanthus roseus].Zhongguo Zhong Yao Za Zhi. 2016 Nov;41(22):4129-4137. doi: 10.4268/cjcmm20162208. Zhongguo Zhong Yao Za Zhi. 2016. PMID: 28933078 Review. Chinese.
Cited by
-
Methylation of sulfhydryl groups: a new function for a family of small molecule plant O-methyltransferases.Plant J. 2006 Apr;46(2):193-205. doi: 10.1111/j.1365-313X.2006.02680.x. Plant J. 2006. PMID: 16623883 Free PMC article.
-
A virus-induced gene silencing approach to understanding alkaloid metabolism in Catharanthus roseus.Phytochemistry. 2011 Nov;72(16):1969-77. doi: 10.1016/j.phytochem.2011.07.001. Epub 2011 Jul 27. Phytochemistry. 2011. PMID: 21802100 Free PMC article.
-
The leaf epidermome of Catharanthus roseus reveals its biochemical specialization.Plant Cell. 2008 Mar;20(3):524-42. doi: 10.1105/tpc.107.056630. Epub 2008 Mar 7. Plant Cell. 2008. PMID: 18326827 Free PMC article.
-
Total synthesis of (-)- and ent-(+)-vindoline and related alkaloids.J Am Chem Soc. 2006 Aug 16;128(32):10596-612. doi: 10.1021/ja061256t. J Am Chem Soc. 2006. PMID: 16895428 Free PMC article.
-
Characterization of a novel N-methyltransferase (NMT) from Catharanthus roseus plants : Detection of NMT and other enzymes of the indole alkaloid biosynthetic pathway in different cell suspension culture systems.Plant Cell Rep. 1987 Dec;6(6):458-61. doi: 10.1007/BF00272782. Plant Cell Rep. 1987. PMID: 24248932