Cascade reactions in multicompartmentalized polymersomes

Angew Chem Int Ed Engl. 2014 Jan 3;53(1):146-50. doi: 10.1002/anie.201308141. Epub 2013 Nov 19.


Enzyme-filled polystyrene-b-poly(3-(isocyano-L-alanyl-aminoethyl)thiophene) (PS-b-PIAT) nanoreactors are encapsulated together with free enzymes and substrates in a larger polybutadiene-b-poly(ethylene oxide) (PB-b-PEO) polymersome, forming a multicompartmentalized structure, which shows structural resemblance to the cell and its organelles. An original cofactor-dependent three-enzyme cascade reaction is performed, using either compatible or incompatible enzymes, which takes place across multiple compartments.

Keywords: artificial cells; cascade reactions; enzyme catalysis; nanoreactors; polymersomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalysis
  • Polymers / chemistry*
  • Polystyrenes / chemistry*


  • Polymers
  • Polystyrenes