Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles

Douglas Roberto Monteiro; Melyssa Negri; Sónia Silva; Luiz Fernando Gorup; Emerson Rodrigues de Camargo; Rosário Oliveira; Debora Barros Barbosa; Mariana Henriques

doi:10.1016/j.colsurfb.2013.10.027

Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles

Colloids Surf B Biointerfaces. 2014 Feb 1:114:410-2. doi: 10.1016/j.colsurfb.2013.10.027. Epub 2013 Oct 30.

Authors

Douglas Roberto Monteiro¹, Melyssa Negri², Sónia Silva³, Luiz Fernando Gorup⁴, Emerson Rodrigues de Camargo⁴, Rosário Oliveira³, Debora Barros Barbosa⁵, Mariana Henriques⁶

Affiliations

¹ Department of Dental Materials and Prosthodontics, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Araçatuba 16015-050, São Paulo, Brazil; Institute for Biotechnology and Bioengineering, Department of Biological Engineering, University of Minho, Braga 4710-057, Portugal.
² Institute for Biotechnology and Bioengineering, Department of Biological Engineering, University of Minho, Braga 4710-057, Portugal; Faculdade INGÁ, Maringá 87070-000, Paraná, Brazil.
³ Institute for Biotechnology and Bioengineering, Department of Biological Engineering, University of Minho, Braga 4710-057, Portugal.
⁴ LIEC-Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos 13565-905, São Paulo, Brazil.
⁵ Department of Dental Materials and Prosthodontics, Araçatuba Dental School, Univ Estadual Paulista (UNESP), Araçatuba 16015-050, São Paulo, Brazil.
⁶ Institute for Biotechnology and Bioengineering, Department of Biological Engineering, University of Minho, Braga 4710-057, Portugal. Electronic address: mcrh@deb.uminho.pt.

PMID: 24257686
DOI: 10.1016/j.colsurfb.2013.10.027

Abstract

This study investigated the adhesion to human epithelial cells and polystyrene surface of viable yeasts recovered from Candida biofilms treated with silver nanoparticles (SN). Biofilm resuspended Candida cells were added to HeLa cells or to empty wells of microtiter plates and the adhesion was verified using crystal violet staining. The adhesion of Candida cells was significantly reduced, mainly when biofilms were pretreated with 54 μg/mL SN. These new findings allow to conclude that SN may induce changes in viable yeasts, which can decrease the dissemination of Candida infections, mainly in susceptible patients.

Keywords: Candida; Epithelial cells; Polystyrene; Silver nanoparticles; Yeast adhesion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biofilms / drug effects*
Candida / cytology*
Candida / drug effects
Candida / physiology*
Cell Adhesion / drug effects
Cell Survival / drug effects
Epithelial Cells / cytology*
Epithelial Cells / drug effects
HeLa Cells
Humans
Metal Nanoparticles / chemistry*
Microbial Viability / drug effects
Polystyrenes / pharmacology*
Silver / pharmacology*

Substances

Polystyrenes
Silver