Adhesion of Candida biofilm cells to human epithelial cells and polystyrene after treatment with silver nanoparticles

Colloids Surf B Biointerfaces. 2014 Feb 1:114:410-2. doi: 10.1016/j.colsurfb.2013.10.027. Epub 2013 Oct 30.


This study investigated the adhesion to human epithelial cells and polystyrene surface of viable yeasts recovered from Candida biofilms treated with silver nanoparticles (SN). Biofilm resuspended Candida cells were added to HeLa cells or to empty wells of microtiter plates and the adhesion was verified using crystal violet staining. The adhesion of Candida cells was significantly reduced, mainly when biofilms were pretreated with 54 μg/mL SN. These new findings allow to conclude that SN may induce changes in viable yeasts, which can decrease the dissemination of Candida infections, mainly in susceptible patients.

Keywords: Candida; Epithelial cells; Polystyrene; Silver nanoparticles; Yeast adhesion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biofilms / drug effects*
  • Candida / cytology*
  • Candida / drug effects
  • Candida / physiology*
  • Cell Adhesion / drug effects
  • Cell Survival / drug effects
  • Epithelial Cells / cytology*
  • Epithelial Cells / drug effects
  • HeLa Cells
  • Humans
  • Metal Nanoparticles / chemistry*
  • Microbial Viability / drug effects
  • Polystyrenes / pharmacology*
  • Silver / pharmacology*


  • Polystyrenes
  • Silver