Production of haploid plantlets in anther cultures of Albizzia lebbeck L

P K Gharyal; A Rashid; S C Maheshwari

doi:10.1007/BF00270188

Production of haploid plantlets in anther cultures of Albizzia lebbeck L

Plant Cell Rep. 1983 Dec;2(6):308-9. doi: 10.1007/BF00270188.

Authors

P K Gharyal¹, A Rashid, S C Maheshwari

Affiliation

¹ Department of Botany, University of Delhi, 110007, Delhi, India.

PMID: 24258192
DOI: 10.1007/BF00270188

Abstract

Anthers of Albizzia lebbeck on B5 medium (BM) supplemented with kinetin (2 mg/l) and 2, 4-D (0.5 mg/l) showed callus initiation from microspores. Differentiation of embryoids and shoots was obtained on BM + BAP (1 mg/l) + IAA (0.5 mg/l) and of roots on BM. Root tip squashes of the regenerated plantlets showed the haploid chromosome number (n=13), confirming the microspore origin of the regenerants.