Comparative N-glycoproteomic and phosphoproteomic profiling of human placental plasma membrane between normal and preeclampsia pregnancies with high-resolution mass spectrometry

PLoS One. 2013 Nov 15;8(11):e80480. doi: 10.1371/journal.pone.0080480. eCollection 2013.


Preeclampsia is a serious complication of pregnancy, which affects 2-8% of all pregnancies and is one of the leading causes of maternal and perinatal mortality and morbidity worldwide. To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used high-resolution LC-MS/MS technologies to construct a comparative N-glycoproteomic and phosphoproteomic profiling of human placental plasma membrane in normal and preeclamptic pregnancies. A total of 1027 N-glyco- and 2094 phospho- sites were detected in human placental plasma membrane, and 5 N-glyco- and 38 phospho- proteins, respectively, with differentially expression were definitively identified between control and preeclamptic placental plasma membrane. Further bioinformatics analysis indicated that these differentially expressed proteins correlate with several specific cellular processes occurring during pathological changes of preeclamptic placental plasma membrane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / metabolism
  • Chorion / metabolism*
  • Computational Biology
  • Endoglin
  • Female
  • Glycoproteins / metabolism*
  • Humans
  • Immunohistochemistry
  • Phosphoproteins / metabolism*
  • Placenta / metabolism*
  • Placenta / pathology
  • Pre-Eclampsia / metabolism*
  • Pre-Eclampsia / pathology
  • Pregnancy
  • Proteome*
  • Proteomics / methods
  • Receptors, Cell Surface / metabolism
  • Tandem Mass Spectrometry


  • Antigens, CD
  • ENG protein, human
  • Endoglin
  • Glycoproteins
  • Phosphoproteins
  • Proteome
  • Receptors, Cell Surface

Grants and funding

This work was supported by the National Natural Science Foundation of China (Grants 21175122 and 91127036) and Technology Development Key Project Fund of Nanjing Medical University (Grants 2011NJMU269 and 2011NJMU270). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.