Ethanol regulates calcium channels in clonal neural cells

Proc Natl Acad Sci U S A. 1986 Aug;83(16):6213-5. doi: 10.1073/pnas.83.16.6213.

Abstract

The acute and long-term effects of ethanol on voltage-dependent Ca channel function were studied in PC12, a clonal cell line of neural crest origin. Acute exposure to ethanol produced a concentration-dependent decrease in depolarization-evoked 45Ca2+ uptake, while prolonged (2-10 days) exposure led to a reciprocal increase in 45Ca2+ uptake and in the number of Ca-channel binding sites labeled by the dihydropyridine Ca-channel antagonist [3H]nitrendipine. Uptake was restored to control levels following withdrawal of ethanol from cultures. These findings indicate that cellular adaptation to ethanol may involve enhanced expression of dihydropyridine-sensitive, voltage-dependent Ca channels.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biological Transport, Active / drug effects
  • Calcium / metabolism*
  • Cell Line
  • Clone Cells
  • Ethanol / pharmacology*
  • Ion Channels / drug effects
  • Ion Channels / physiology*
  • Kinetics
  • Neural Crest / drug effects
  • Neural Crest / physiology*
  • Potassium / pharmacology

Substances

  • Ion Channels
  • Ethanol
  • Potassium
  • Calcium