Platelet-derived microparticles are implicated in remote ischemia conditioning in a rat model of cerebral infarction

CNS Neurosci Ther. 2013 Dec;19(12):917-25. doi: 10.1111/cns.12199. Epub 2013 Nov 4.


Aim: Remote ischemic preconditioning protects against ischemic organ damage by giving short periods of subcritical ischemia to a remote organ. We tested the hypothesis that remote ischemic conditioning can attenuate cerebral stroke in a rat middle cerebral artery occlusion (MCAO) model by microparticles (MPs).

Methods and results: MPs were extracted from healthy rats that underwent hindlimb ischemia-reperfusion preconditioning (RIPC), and were transfused into rats that had undergone MCAO without RIPC. The transfusion resulted in an increase in platelet-derived MPs in blood and reduction in infarction area, confirmed by both 2-3-5-triphenyltetrazolium chloride staining and magnetic resonance imaging, albeit to a lesser degree than RIPC itself. Behavioral tests (modified Neurological Severity Score [mNSS]) were calculated to judge the behavioral change. However, no significant difference was observed after MP transfusion in 24 h or the following consecutive 9 days.

Conclusions: RIPC induces an increase in MPs, and platelet-derived MPs may confer at least part of the remote protective effect against cerebral ischemic-reperfusion injury.

Keywords: Ischemic stroke; Microparticles; Per-conditioning; Remote ischemic preconditioning.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell-Derived Microparticles / pathology*
  • Cell-Derived Microparticles / ultrastructure
  • Cerebral Infarction / etiology*
  • Cerebral Infarction / prevention & control*
  • Disease Models, Animal
  • Endothelial Cells / pathology
  • Flow Cytometry
  • Infarction, Middle Cerebral Artery / complications*
  • Ischemic Preconditioning*
  • Male
  • Microscopy, Electron, Transmission
  • Nervous System Diseases / diagnosis
  • Nervous System Diseases / etiology
  • Platelet Membrane Glycoprotein IIb / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Upper Extremity / physiopathology


  • Platelet Membrane Glycoprotein IIb