A proteomic study of rice cultivar TNG67 and its high aroma mutant SA0420

Plant Sci. 2014 Jan;214:20-8. doi: 10.1016/j.plantsci.2013.09.010. Epub 2013 Sep 27.

Abstract

Fragrance is a very important economic trait for rice cultivars. To identify the aroma genes in rice, we performed a proteomics analysis of aroma-related proteins between Tainung 67 (TNG67) and its high aroma mutant SA0420. Seventeen of the differentially identified proteins were close related with the aroma phenotype of SA0420. Among them, 9 were found in leaves and 8 were found in grains. One protein (L3) was identified as the chloroplastic glyceraldehyde-3-phosphate dehydrogenase B (OsGAPDHB) which was less abundant in SA0420 than TNG67. Sequence analysis demonstrated that this protein in SA0420 carries a P425S mutation in the C-terminal extension domain, which might hinder the formation of holoenzyme, thereby changing the profile of aroma compounds. The protein profile of OsGAPDHB showed only a weak correlation to its transcription profile. This result indicated that the reduction of OsGAPDHB in SA0420 is regulated by post-translational processes and can only be analyzed by proteomics approach. Transgenic lines suppressing OsGAPDHB through RNAi harbored more fragrance than TNG67 but less than SA0420. With betaine-aldehyde dehydrogenase as the only fragrance gene identified in rice to date, OsGAPDHB may serve as the second protein known to contribute to the aroma phenotype.

Keywords: 2-Acetyl-1-pyrroline; 2-D Gel Electrophoresis; Aroma rice; Differential display; Glyceraldehyde-3-phosphate dehydrogenase; Oryza sativa L..

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Chloroplast Proteins / genetics
  • Chloroplast Proteins / metabolism
  • Electrophoresis, Gel, Two-Dimensional
  • Gene Expression Regulation, Plant
  • Glyceraldehyde-3-Phosphate Dehydrogenases / classification
  • Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
  • Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
  • Mutation*
  • Odorants*
  • Oryza / genetics
  • Oryza / metabolism*
  • Phylogeny
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plants, Genetically Modified
  • Protein Processing, Post-Translational
  • Proteomics / methods*
  • RNA Interference
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Chloroplast Proteins
  • Plant Proteins
  • Glyceraldehyde-3-Phosphate Dehydrogenases