Calcitonin gene-related peptide immunoreactive sensory neurons in the vagal and glossopharyngeal ganglia innervating the larynx of the rat

J Chem Neuroanat. 2014 Jan;55:18-23. doi: 10.1016/j.jchemneu.2013.11.001. Epub 2013 Nov 20.

Abstract

We have examined whether calcitonin gene-related peptide-immunoreactive (CGRP-ir) neurons in the vagal and glossopharyngeal ganglia innervate the larynx. Many CGRP-ir neurons were located mostly in the superior glossopharyngeal-jugular ganglion complex that was fused the superior glossopharyngeal ganglion and the jugular ganglion in the cranial cavity. When Fluorogold was applied to the cut end of the superior laryngeal nerve (SLN) or the recurrent laryngeal nerve (RLN), many Fluorogold-labeled neurons were found in the superior glossopharyngeal-jugular ganglion complex and the nodose ganglion. Double-labeling for CGRP and Fluorogold showed that about 80% of Fluorogold-labeled neurons in the superior glossopharyngeal-jugular ganglion complex expressed CGRP-like immunoreactivity in the case of application to the SLN, and about 50% of Fluorogold-labeled neurons expressed CGRP-like immunoreactivity in the case of the RLN. Only a few double-labeled neurons were found in the nodose ganglion. The number of the Fluorogold-labeled neurons and double-labeled neurons in the superior glossopharyngeal-jugular ganglion complex in the case of the SLN was larger than that in the case of the RLN. These results indicate that sensory information from the larynx might be conveyed by many CGRP-ir neurons located in the superior glossopharyngeal-jugular ganglion complex by way of the SLN and the RLN.

Keywords: Cranial ganglia; Immunohistochemistry; Recurrent laryngeal nerve; Retrograde tracing; Superior laryngeal nerve.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcitonin Gene-Related Peptide / metabolism*
  • Ganglia, Sensory / metabolism*
  • Glossopharyngeal Nerve / metabolism*
  • Immunohistochemistry
  • Laryngeal Nerves / metabolism*
  • Larynx / metabolism
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Sensory Receptor Cells / metabolism
  • Vagus Nerve / metabolism*

Substances

  • Calcitonin Gene-Related Peptide