A novel hairpin-like antimicrobial peptide from barnyard grass (Echinochloa crusgalli L.) seeds: Structure-functional and molecular-genetics characterization

Biochimie. 2014 Apr;99:63-70. doi: 10.1016/j.biochi.2013.11.005. Epub 2013 Nov 23.

Abstract

A novel plant hairpin-like defense polypeptide named EcAMP3 was isolated from latent barnyard grass (Echinochloa crusgalli L.) seeds. The native peptide and its recombinant analogue were characterized. EcAMP3 displays antifungal and antibacterial activity in vitro. The gene family encoding EcAMPs precursor protein was also characterized; the genes and pseudogenes of this family show 97-100% homology. Every member of EcAMPs precursor family contains seven identical cysteine motifs: C1XXXC2(11-13)C3XXXC4. One of those motifs corresponds to the isolated peptide. EcAMP3 is the first member of the plant hairpin-like peptide family that inhibits the growth of phytopathogenic bacteria. Obtained results can explain the nature of the complex resistance of barnyard grass to a variety of pathogenic microorganisms.

Keywords: Antibacterial activity; Antifungal activity; Full-length gene structure; Full-size precursor protein; Hairpin-like peptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / drug effects
  • Amino Acid Sequence
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology*
  • Antifungal Agents / chemistry
  • Antifungal Agents / pharmacology*
  • Antimicrobial Cationic Peptides / chemistry
  • Antimicrobial Cationic Peptides / genetics
  • Antimicrobial Cationic Peptides / pharmacology*
  • Aspergillus niger / drug effects
  • Echinochloa / chemistry*
  • Fusarium / drug effects
  • Inhibitory Concentration 50
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Pectobacterium carotovorum / drug effects
  • Plant Diseases / microbiology
  • Plant Extracts / chemistry
  • Plant Extracts / genetics
  • Plant Extracts / pharmacology
  • Plant Immunity
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / pharmacology*
  • Protein Structure, Secondary
  • Pseudomonas syringae / drug effects
  • Seeds / chemistry*
  • Sequence Homology, Amino Acid

Substances

  • Anti-Bacterial Agents
  • Antifungal Agents
  • Antimicrobial Cationic Peptides
  • Plant Extracts
  • Plant Proteins