The development of NAD(P)H-dependent and ferredoxin-dependent glutamate synthase in greening barley and pea leaves

R M Wallsgrove; P J Lea; B J Miflin

doi:10.1007/BF01267816

The development of NAD(P)H-dependent and ferredoxin-dependent glutamate synthase in greening barley and pea leaves

Planta. 1982 Sep;154(5):473-6. doi: 10.1007/BF01267816.

Authors

R M Wallsgrove¹, P J Lea, B J Miflin

Affiliation

¹ Department of Biochemistry, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK.

PMID: 24276277
DOI: 10.1007/BF01267816

Abstract

The activity of NAD(P)H-dependent glutamate synthase (E.C. 1.4.1.14) has been demonstrated in extracts from etiolated shoots of pea (Pisum sativum L.) and barley (Hordeum vulgare L.). This activity does not significantly alter upon greening of the etiolated shoots, and is at a similar level in light-grown material. Ferredoxin-dependent glutamate synthase (E.C. 1.4.7.1) has low activity in etiolated shoots but increases rapidly on greening. In light grown leaves ferredoxin-dependent activity is 30-40-fold higher than NAD(P)H-dependent activity. It is not considered that the NAD(P)H-dependent glutamate synthase plays an important role in ammonia assimilation in the photosynthetic tissue of higher plants.