Contribution of β-lactamases and porin proteins OmpK35 and OmpK36 to carbapenem resistance in clinical isolates of KPC-2-producing Klebsiella pneumoniae

Antimicrob Agents Chemother. 2014;58(2):1214-7. doi: 10.1128/AAC.02045-12. Epub 2013 Nov 25.

Abstract

Fifty-seven carbapenem-resistant Klebsiella pneumoniae isolates belonging to ST11 (50 isolates), ST423 (5 isolates), and two other sequence types were studied. All were positive for blaKPC-2, blaTEM-1, and blaCTX-M-14. SDS-PAGE analysis of six representative isolates demonstrated varied porin expression. Nevertheless, when blaKPC-2 was deleted, carbapenem resistance was markedly reduced. Additionally, SHV-12, DHA-1, and/or VIM-1 appeared to contribute to accessory carbapenemase activity. In contrast, OmpK35 and/or OmpK36 deficiency seemed to serve only as a minor cooperative factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Carbapenems / pharmacology
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation, Bacterial*
  • Humans
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / enzymology
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Molecular Sequence Data
  • Plasmids
  • Porins / genetics*
  • Porins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carbapenems
  • OmpK35 porin, Klebsiella pneumoniae
  • OmpK36 protein, Klebsiella pneumoniae
  • Porins
  • VIM-1 metallo-beta-lactamase
  • beta-lactamase SHV-12
  • beta-Lactamases
  • beta-lactamase KPC-2, Klebsiella pneumoniae

Associated data

  • GENBANK/JX276743
  • GENBANK/JX291113
  • GENBANK/JX291114
  • GENBANK/JX310550
  • GENBANK/JX310551
  • GENBANK/JX310552
  • GENBANK/JX310553
  • GENBANK/JX310554
  • GENBANK/JX310555
  • GENBANK/JX310556
  • GENBANK/JX310557