Molecular cloning and characterisation of a novel type of human papillomavirus 160 isolated from a flat wart of an immunocompetent patient

PLoS One. 2013 Nov 8;8(11):e79592. doi: 10.1371/journal.pone.0079592. eCollection 2013.

Abstract

More than 150 types of Human papillomaviruses (HPVs) have been isolated from numerous cutaneous and/or mucosal lesions. Flat wart samples on the face from 36 immunocompetent patients were collected and screened for HPV. From one sample, we cloned a putative novel genotype. The novel type consisted of 7779 bp in length with a GC content of 47.1%, containing open reading frames for putative early proteins (E1, E2, E4, E6, and E7) and two late proteins (L1 and L2). Homology searches and phylogenetic analyses indicated that it belonged to Alphapapillomavirus (Alpha-PV) species 2 and most closely resembled HPV 3. The virus fulfilled the definition of a novel type, and was named HPV 160 by the Reference Center for Papillomaviruses. The putative E7 protein of HPV 160 as well as HPV 29, 77, and 78 contained the Leu-X-Cys-X-Glu pRB-binding motif but other Alpha-PV species 2 (HPV 3, 10, 28, 94, 117, and 125) did not have this conserved motif.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Alphapapillomavirus / classification
  • Alphapapillomavirus / genetics*
  • Alphapapillomavirus / isolation & purification
  • Amino Acid Sequence
  • Binding Sites
  • Child
  • Cloning, Molecular
  • DNA, Viral / chemistry
  • Genome, Viral
  • Genotype
  • Humans
  • Immunocompetence
  • Middle Aged
  • Open Reading Frames
  • Papillomavirus Infections / pathology
  • Papillomavirus Infections / virology*
  • Phylogeny
  • Sequence Alignment
  • Sequence Analysis, Protein
  • Viral Proteins / chemistry
  • Viral Proteins / genetics
  • Warts / pathology
  • Warts / virology*

Substances

  • DNA, Viral
  • Viral Proteins

Grant support

The authors have no support or funding to report.