The diagnostic value of CSF α-synuclein in the differential diagnosis of dementia with Lewy bodies vs. normal subjects and patients with Alzheimer's disease

PLoS One. 2013 Nov 25;8(11):e81654. doi: 10.1371/journal.pone.0081654. eCollection 2013.

Abstract

The detection of α-synuclein (α-syn) in the cerebrospinal fluid (CSF) of patients with synucleinopathy has yielded promising but inconclusive results. The aim of the present study was to determine the diagnostic value of α-syn as a biological marker for Dementia with Lewy bodies (DLB) vs. normal subjects and patients with Alzheimer's disease (AD), after strict control of several recognized confounders. Sixteen patients with DLB, 18 patients with AD and 22 age- and sex-matched normal controls (CTRL) were recruited. The levels of total α-syn in CSF were measured using a novel enzyme-linked immunosorbent assay. There was a significant increase of CSF α-syn levels in DLB patients as compared to the CTRL and AD groups (P = 0.049 and 0.01 respectively). ROC analysis revealed that increased α-syn was 81.8% specific for the discrimination of DLB vs. CTRL and 90% vs. AD. However, sensitivity was lower (56.2 % and 50% respectively). These findings provide evidence for a possible diagnostic role of α-syn as a surrogate biomarker for DLB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Alzheimer Disease / cerebrospinal fluid
  • Alzheimer Disease / diagnosis*
  • Biomarkers / cerebrospinal fluid*
  • Case-Control Studies
  • Diagnosis, Differential
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Lewy Body Disease / cerebrospinal fluid
  • Lewy Body Disease / diagnosis*
  • Middle Aged
  • Sensitivity and Specificity
  • Spinal Puncture
  • alpha-Synuclein / cerebrospinal fluid*

Substances

  • Biomarkers
  • alpha-Synuclein

Grant support

The project presented here is part of the BIOMARKAPD Project in the frame of the Joint Programming Neurodegenerative Disease. This work was partly funded by a grant from the MJF Foundation to EE and KV. No additional external funding was received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.