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Comment
. 2013 Dec 10;110(50):19980-1.
doi: 10.1073/pnas.1319939110. Epub 2013 Nov 27.

Dual Defensin Strategy for Targeting Enterococcus Faecalis

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Dual Defensin Strategy for Targeting Enterococcus Faecalis

Michael S Gilmore et al. Proc Natl Acad Sci U S A. .
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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
(A) New M protein deposited on the cell wall of M type 19 group A Streptococcus. Preexisting M protein was blocked using unlabeled antibody. After 30-min additional growth, fluorescein-labeled anti-M19 antibody stains the growing chain mainly near the septa of dividing cells. Blue-dashed cartoon lines depict approximate inferred cell boundaries, added for illustration (adapted from ref. 7). (Magnification, 2,100×.) (B) S. pyogenes, grown first in the presence of trypsin and pronase to removed preformed surface proteins, then subcultured for an additional 2 min before fixing and staining to label newly integrated cell wall proteins. Cells were stained with fluorescent antibody to M protein (red) and protein F (green), highlighting the differential initial localization of the YSIRK-signal peptide containing M protein at the septum (adapted from ref. 11). (C) Transient focal location of sortase (red) at or near the septum (green) at early stages in cell division of S. pyogenes (adapted from ref. 15). In early phases of S. pyogenes growth, sortase largely localizes to an area coincident with the septum. At later stages in cell division, SrtA appears to distribute more evenly around the cell, essentially as found in the accompanying examination of sortase distribution in E. faecalis (6).

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