Monoclonal antibody G 250 recognizes a determinant present in renal-cell carcinoma and absent from normal kidney

Int J Cancer. 1986 Oct 15;38(4):489-94. doi: 10.1002/ijc.2910380406.


This report describes a monoclonal antibody designated G 250, subclass IgGl, that recognizes an antigen preferentially expressed on cell membranes of renal-cell carcinoma cells (RCC) and not expressed in normal proximal tubular epithelium. G 250 antibody reacted with 46 of 47 primary RCC, with 7 of 8 RCC metastases and with a few other malignant tumors. The staining pattern of G 250 differs from that of other RCC-related antibodies described. Preliminary experiments show that this antibody can be used to visualize RCC xenografts in nude mice by immunoscintigraphy.

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Carcinoma, Renal Cell / immunology*
  • Cell Line
  • Enzyme-Linked Immunosorbent Assay
  • Epithelium / immunology
  • Epitopes / immunology*
  • Humans
  • Kidney / immunology*
  • Kidney Tubules, Proximal / cytology
  • Kidney Tubules, Proximal / immunology
  • Mice
  • Mice, Nude
  • Radionuclide Imaging
  • Tissue Distribution
  • Transplantation, Heterologous


  • Antibodies, Monoclonal
  • Epitopes