Abstract
Although oxalic acid is common in nature our understanding of the mechanism(s) regulating its turnover remains incomplete. In this study we identify Saccharomyces cerevisiae acyl-activating enzyme 3 (ScAAE3) as an enzyme capable of catalyzing the conversion of oxalate to oxalyl-CoA. Based on our findings we propose that ScAAE3 catalyzes the first step in a novel pathway of oxalate degradation to protect the cell against the harmful effects of oxalate derived from an endogenous process or an environmental source.
Keywords:
Acyl-activating; Degradation; Oxalate; Oxalyl-CoA.
Published by Elsevier B.V.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Acyl Coenzyme A / metabolism*
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Carbon Dioxide / metabolism
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Coenzyme A Ligases / genetics
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Coenzyme A Ligases / metabolism*
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Gene Expression Regulation, Developmental
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Gene Expression Regulation, Fungal
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Hydrogen-Ion Concentration
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Kinetics
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Models, Biological
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Mutation
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Oxalates / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
Substances
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Acyl Coenzyme A
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Oxalates
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Saccharomyces cerevisiae Proteins
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oxalyl-coenzyme A
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Carbon Dioxide
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Coenzyme A Ligases
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oxalyl-CoA synthetase, S cerevisiae