Advantages of monodisperse and chemically robust "SpheriCal" polyester dendrimers as a "universal" MS calibrant

J Am Soc Mass Spectrom. 2014 Mar;25(3):303-9. doi: 10.1007/s13361-013-0777-8. Epub 2013 Dec 3.


The utilization of dendrimer calibrants as an alternative to peptides and proteins for high mass calibration is explored. These synthetic macromolecules exhibited a number of attractive advantages, including exceptional shelf-lives, broad compatibility with a wide range of matrices and solvents, and evenly spaced calibration masses across the mass range examined, 700-30,000 u. The exceptional purity of these dendrimers and the technical simplicity of this calibration platform validate their broad relevance for high molecular weight mass spectrometry.

MeSH terms

  • Calibration / standards
  • Dendrimers / chemistry*
  • Models, Molecular
  • Molecular Weight
  • Polyesters / chemistry*
  • Reference Standards
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / standards*


  • Dendrimers
  • Polyesters