Re-scan confocal microscopy: scanning twice for better resolution

Biomed Opt Express. 2013 Oct 25;4(11):2644-56. doi: 10.1364/BOE.4.002644. eCollection 2013.


We present a new super-resolution technique, Re-scan Confocal Microscopy (RCM), based on standard confocal microscopy extended with an optical (re-scanning) unit that projects the image directly on a CCD-camera. This new microscope has improved lateral resolution and strongly improved sensitivity while maintaining the sectioning capability of a standard confocal microscope. This simple technology is typically useful for biological applications where the combination high-resolution and high-sensitivity is required.

Keywords: (110.2990) Image formation theory; (170.5810) Scanning microscopy; (170.6900) Three-dimensional microscopy; (180.1790) Confocal microscopy; (350.5730) Resolution.