The ATP-sensitivity of K+ channels in rat pancreatic B-cells is modulated by ADP

FEBS Lett. 1986 Nov 10;208(1):63-6. doi: 10.1016/0014-5793(86)81533-2.

Abstract

ATP-sensitive K+ channels in inside-out membrane patches from dispersed rat pancreatic B-cells were studied using patch-clamp methods. The dose-response curve for ATP-induced channel inhibition was shifted to higher concentrations in the presence of ADP (2 mM). In glucose-free solution, the total intracellular concentration of ATP was 3.8 mM and of ADP 1.5 mM; glucose (20 mM) increased ATP and decreased ADP by approx. 40%. These results suggest that both ADP and ATP may be involved in regulating the activity of the glucose-sensitive K+ channel in intact B-cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Diphosphate / metabolism
  • Adenosine Diphosphate / physiology*
  • Adenosine Triphosphate / metabolism
  • Adenosine Triphosphate / physiology*
  • Animals
  • Glucose / pharmacology
  • In Vitro Techniques
  • Ion Channels / physiology*
  • Islets of Langerhans / physiology*
  • Potassium / metabolism
  • Rats

Substances

  • Ion Channels
  • Adenosine Diphosphate
  • Adenosine Triphosphate
  • Glucose
  • Potassium