Direct identification of small sequence changes in chromosomal DNA

J M Huibregtse; D R Engelke

doi:10.1016/0378-1119(86)90056-9

Direct identification of small sequence changes in chromosomal DNA

Gene. 1986;44(1):151-8. doi: 10.1016/0378-1119(86)90056-9.

Authors

J M Huibregtse, D R Engelke

PMID: 2429900
DOI: 10.1016/0378-1119(86)90056-9

Abstract

Dideoxynucleotide chain termination sequencing has been applied directly to genomic DNA templates by annealing radiolabeled oligodeoxynucleotide primers to unique sites in total yeast DNA and extending with avian myoblastosis virus (AMV) reverse transcriptase. The technique is used here to confirm the introduction of selectively altered tRNA genes into the Saccharomyces cerevisiae genome by gene replacement.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Base Sequence
Chromosomes / analysis*
Cloning, Molecular
DNA, Fungal / genetics*
Genes, Fungal*
RNA, Transfer, Amino Acyl / genetics*
RNA-Directed DNA Polymerase
Saccharomyces cerevisiae / genetics*

Substances

DNA, Fungal
RNA, Transfer, Amino Acyl
RNA-Directed DNA Polymerase