Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Feb;281(4):1057-67.
doi: 10.1111/febs.12668. Epub 2014 Jan 15.

Interaction of Human Minichromosome Maintenance Protein-Binding Protein With Minichromosome Maintenance 2-7

Affiliations
Free article

Interaction of Human Minichromosome Maintenance Protein-Binding Protein With Minichromosome Maintenance 2-7

Shunsuke Kusunoki et al. FEBS J. .
Free article

Abstract

It has been reported that minichromosome maintenance protein-binding protein (MCM-BP) functions in the formation of the pre-replication complex, unloading of minichromosome maintenance (MCM)2-7 from chromatin in late S phase, and formation of the cohesion complex by interacting with MCM3-7 proteins, suggesting that MCM-BP functions in several different reactions during the cell cycle. Here, we examined the interaction of human MCM-BP with MCM2-7 and structural maintenance of chromosome 3 in synchronized HeLa cells by immunoprecipitation. The results show that MCM-BP mainly interacts with MCM7 in the Triton-soluble fraction from S phase and G(2) phase cells, and it also interacts with structural maintenance of chromosome 3 in the fraction from G(2) phase cells. In vitro studies show that MCM-BP disassembles MCM2-7 bound to DNA with a fork-like structure by interacting with MCM3, MCM5, and MCM7. These results suggest that MCM-BP functions in disassembling MCM2-7 on chromatin during S phase and G2 phase by interacting with MCM3, MCM5, and MCM7.

Keywords: DNA replication fork; minichromosome maintenance protein-binding protein; minichromosome maintenance 2-7; protein interaction; structural maintenance of chromosome.

Similar articles

See all similar articles

Cited by 2 articles

Publication types

MeSH terms

Substances

LinkOut - more resources

Feedback