Nociceptive behavior after intrathecal injections of substance P, neurokinin A and calcitonin gene-related peptide in mice

Neurosci Lett. 1986 Sep 25;70(1):143-7. doi: 10.1016/0304-3940(86)90453-2.


Intrathecal injections of substance P (SP) or neurokinin A (NKA) in the mouse caused dose-dependent reciprocal hindlimb scratching, licking and biting responses directed to the caudal part of the body. NKA decreased the latency in the tail flick assay but like SP, did not alter the reactions in the hot plate test or the hypertonic saline assay. Although immunoreactivity of calcitonin gene-related peptide (CGRP) was detected in mouse spinal cord, CGRP caused no behavioral reactions, nor did it significantly affect thermo- or chemonociception or the scratching induced by SP. Since NKA-like immunoreactivity was found to be present in sensory neurons, NKA as well as SP are likely transmitters of nociceptive primary afferent neurons.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcitonin Gene-Related Peptide
  • Female
  • Injections, Spinal
  • Mice
  • Neurokinin A
  • Neuropeptides / analysis
  • Neuropeptides / pharmacology*
  • Pain / chemically induced*
  • Peripheral Nerves / analysis
  • Spinal Cord / analysis
  • Spinal Cord / drug effects*
  • Substance P / pharmacology*
  • Thermosensing / drug effects


  • Neuropeptides
  • Substance P
  • Neurokinin A
  • Calcitonin Gene-Related Peptide