Inhibition of angiogenesis by the antineoplastic agents mitoxantrone and bisantrene

Biochem Biophys Res Commun. 1986 Nov 14;140(3):901-7. doi: 10.1016/0006-291x(86)90720-5.


The effects of mitoxantrone and bisantrene on angiogenic responses induced by tumor cell-conditioned media in the avascular cornea of rat eye have been evaluated. Both mitoxantrone and bisantrene effectively inhibited, in a concentration-dependent manner, angiogenesis induced by conditioned media obtained from either a hamster buccal pouch carcinoma cell line or P388D1 murine macrophage-like cells. Whereas vessel ingrowth in corneas containing tumor cell-conditioned media was detected as early as day 2 or 3 and was maximal by day 7, inclusion of mitoxantrone or bisantrene in the conditioned media at a 1:1 ratio (160 microM mitoxantrone or 32 microM bisantrene) resulted in complete inhibition of angiogenesis throughout the 14-day evaluation period. When concentrations of 64 and 32 microM mitoxantrone or 13 and 6.4 microM bisantrene were employed there was a marked delay in the appearance of capillary blood vessels (day 5 to 7) and a reduction in the intensity of angiogenic responses. No untoward toxicity to the tissue was observed at the concentrations of mitoxantrone or bisantrene employed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anthracenes / pharmacology
  • Antineoplastic Agents / pharmacology*
  • Cornea / blood supply
  • Culture Media
  • Mitoxantrone / pharmacology*
  • Neovascularization, Pathologic / physiopathology*
  • Rats


  • Anthracenes
  • Antineoplastic Agents
  • Culture Media
  • bisantrene
  • Mitoxantrone