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, 47 (5), 649-56

Transfection of a DNA Locus That Mediates the Conversion of 10T1/2 Fibroblasts to Myoblasts

Transfection of a DNA Locus That Mediates the Conversion of 10T1/2 Fibroblasts to Myoblasts

A B Lassar et al. Cell.

Abstract

Stable myoblast cell lines were isolated after a brief exposure of mouse fibroblasts (10T1/2 cells) to 5-azacytidine. We show that transfection of 10T1/2 cells with DNA from these azacytidine-induced myoblasts (or from mouse C2C12 myoblasts) results in myogenic conversion of approximately 1 in 15,000 transfected colonies. In contrast, transfection of 10T1/2 cells with DNA from nonmyogenic cells (parental 10T1/2 cell DNA) does not give rise to myoblast colonies. These results indicate that an azacytidine-induced structural modification (presumably demethylation) in the DNA of a single locus is sufficient to convert 10T1/2 cells into determined myoblasts.

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