Structure and properties of the C-terminal β-helical domain of VgrG protein from Escherichia coli O157

J Biochem. 2014 Mar;155(3):173-82. doi: 10.1093/jb/mvt109. Epub 2013 Dec 3.


The bacterial Type 6 secretion system (T6SS) translocates protein toxins (also called effectors) from the cytosol of a T6SS-carrying cell to a target cell by a syringe-like supramolecular complex resembling a contractile tail of bacteriophages. Valine-glycine repeat protein G (VgrG) proteins, which are the homologues of the gp27-gp5 (gene product) cell puncturing complex of bacteriophage T4, are considered to be located at the attacking tip of the bacterial T6SS apparatus. Here, we over-expressed six VgrG proteins from pathogenic Escherichia coli O157 and CFT073 strains. Purified VgrG1 of E. coli O157 and c3393 of E. coli CFT073 form trimer in solution and are rich in β-structure. We also solved the crystal structure of a trypsin-resistant C-terminal fragment of E. coli O157 VgrG1 (VgrG1C(G561)) at 1.95 Å resolution. VgrG1C(G561) forms a three-stranded antiparallel β-helix which is structurally similar to the β-helix domain of the central spike protein (gp138) of phi92 phage, indicating a possible evolutional relationship. Comparison of four different three-stranded β-helix proteins shows how their amino acid composition determines the protein fold.

Keywords: Type 6 secretion system; X-ray crystallography; bacteria; structure; three-stranded β-helix.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Crystallography, X-Ray
  • Escherichia coli O157 / isolation & purification
  • Escherichia coli O157 / metabolism*
  • Escherichia coli Proteins / chemistry*
  • Escherichia coli Proteins / metabolism*
  • Hydrogen Bonding
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Multimerization
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Proteolysis
  • Salts / metabolism
  • Solutions
  • Structure-Activity Relationship
  • Trypsin / metabolism


  • Escherichia coli Proteins
  • Salts
  • Solutions
  • Trypsin

Associated data

  • PDB/3WIT