Thiazole orange: a new dye for reticulocyte analysis

Cytometry. 1986 Nov;7(6):508-17. doi: 10.1002/cyto.990070603.


The purpose of this study was to find a 488-nm excitable fluorescent dye for reticulocyte analysis by the use of fluorescence activated cell cytometry. The chemical structure of thioflavin T, a dye used for reticulocyte analysis with 457-nm excitation, was used as a model. Several dyes were synthesized and evaluated by quantum yield determination, fluorescence microscopy, and flow cytometry. The best results were obtained with a dye we have named "thiazole orange"; analysis of several blood samples with thiazole orange gave a correlation coefficient of 0.97 as compared to a manual determination of reticulocyte percentage.

Publication types

  • Comparative Study

MeSH terms

  • Benzothiazoles
  • Cell Count
  • DNA / metabolism
  • Flow Cytometry
  • Fluorescent Dyes*
  • Humans
  • Quinolines
  • RNA / metabolism
  • Reticulocytes / cytology
  • Reticulocytes / metabolism*
  • Thiazoles


  • Benzothiazoles
  • Fluorescent Dyes
  • Quinolines
  • Thiazoles
  • thiazole orange
  • thioflavin T
  • RNA
  • DNA