Glyphosate catabolism by Pseudomonas sp. strain PG2982

J Bacteriol. 1986 Nov;168(2):702-7. doi: 10.1128/jb.168.2.702-707.1986.


The pathway for the degradation of glyphosate (N-phosphonomethylglycine) by Pseudomonas sp. PG2982 has been determined by using metabolic radiolabeling experiments. Radiorespirometry experiments utilizing [3-14C]glyphosate revealed that approximately 50 to 59% of the C-3 carbon was oxidized to CO2. Fractionation of stationary-phase cells labeled with [3-14C]glyphosate revealed that from 45 to 47% of the assimilated label is distributed to proteins and that the amino acids methionine and serine are highly labeled. Adenine and guanine received 90% of the C-3 label found in the nucleic acid fraction, and the only pyrimidine base labeled was thymine. These results indicated that C-3 of glyphosate was at some point metabolized to a C-1 compound whose ultimate fate could be both oxidation to CO2 and distribution to amino acids and nucleic acid bases that receive a C-1 group from the C-1-donating coenzyme tetrahydrofolate. Pulse-labeling of PG2982 cells with [3-14C]glyphosate resulted in the isolation of [3-14C]sarcosine as an intermediate in glyphosate degradation. Examination of crude extracts prepared from PG2982 cells revealed the presence of a sarcosine-oxidizing enzyme that oxidizes sarcosine to glycine and formaldehyde. These results indicate that the first step in glyphosate degradation by PG2982 is cleavage of the carbon-phosphorus bond, resulting in the release of sarcosine and a phosphate group. The phosphate group is utilized as a source of phosphorus, and the sarcosine is degraded to glycine and formaldehyde. This pathway is supported by the results of [1,2-14C]glyphosate metabolism studies, which show that radioactivity in the proteins of labeled cells is found only in the glycine and serine residues.

MeSH terms

  • Adenine / metabolism
  • Bacterial Proteins / metabolism
  • Formaldehyde / metabolism
  • Formates / metabolism
  • Glycine / analogs & derivatives*
  • Glycine / metabolism
  • Guanine / metabolism
  • Methionine / metabolism
  • Oxidoreductases, N-Demethylating / metabolism
  • Pseudomonas / enzymology
  • Pseudomonas / metabolism*
  • Sarcosine / metabolism
  • Sarcosine Oxidase
  • Serine / metabolism


  • Bacterial Proteins
  • Formates
  • formic acid
  • Formaldehyde
  • Serine
  • glyphosate
  • Guanine
  • Methionine
  • Oxidoreductases, N-Demethylating
  • Sarcosine Oxidase
  • Adenine
  • Glycine
  • Sarcosine