Myosin 1g regulates cytoskeleton plasticity, cell migration, exocytosis, and endocytosis in B lymphocytes

Eur J Immunol. 2014 Mar;44(3):877-86. doi: 10.1002/eji.201343873. Epub 2014 Jan 16.


Myosin 1g (Myo1g) is a hematopoietic-specific myosin expressed mainly by lymphocytes. Here, we report the localization of Myo1g in B-cell membrane compartments such as lipid rafts, microvilli, and membrane extensions formed during spreading. By using Myo1g-deficient mouse B cells, we detected abnormalities in the adhesion ability and chemokine-induced directed migration of these lymphocytes. We also assessed a role for Myo1g in phagocytosis and exocytosis processes, as these were also irregular in Myo1g-deficient B cells. Taken together, our results show that Myo1g acts as a main regulator of different membrane/cytoskeleton-dependent processes in B lymphocytes.

Keywords: B lymphocyte; Chemotaxis; Cytoskeleton; Exo-endocytosis; Myosin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / metabolism*
  • Cell Adhesion / genetics
  • Cell Movement / genetics*
  • Cell Movement / immunology*
  • Cells, Cultured
  • Cytoskeleton / metabolism*
  • Endocytosis / physiology*
  • Exocytosis / physiology*
  • Female
  • Mice
  • Mice, Knockout
  • Minor Histocompatibility Antigens
  • Myosins / genetics*
  • Myosins / metabolism
  • Protein Transport


  • Minor Histocompatibility Antigens
  • Myo1g protein, mouse
  • Myosins