microRNA expression in hepatitis B virus infected primary treeshrew hepatocytes and the independence of intracellular miR-122 level for de novo HBV infection in culture

Virology. 2014 Jan 5;448:247-54. doi: 10.1016/j.virol.2013.10.016. Epub 2013 Nov 1.

Abstract

Infection of Hepatitis B virus (HBV) in hepatocytes has been known to be controlled by multiple cellular factors, while the relationship of the infection and liver microRNAs remains obscure. In this study, a miRNA database, containing 168 unique mature miRNA members from primary hepatocytes of a primate-like animal, northern treeshrew (Tupaia belangeri) that is the only species susceptible for HBV infection other than human and chimpanzee, was established. The relative level of a liver predominant microRNA, miR-122, was markedly increased upon HBV infection of the primary tupaia hepatocyte (PTH). However, introducing neither miR-122 nor its antagonist anti-miR-122 into PTHs, or, HepG2-NTCP that is HepG2 cells with the newly identified receptor sodium taurocholate cotransporting polypeptide (NTCP) did not alter the viral infection on these cells. These data suggest that de novo HBV infection of cultured hepatocytes does not depend on the expression level of intracellular miR-122 of the target cells.

Keywords: De novo infection; Hepatitis B virus; Hepatocyte; MicroRNA database; MicroRNA-122; Pathogenesis; RNA deep sequencing; Sodium taurocholate cotransporting polypeptide (NTCP); Tupaia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Disease Models, Animal*
  • Hepatitis B / genetics*
  • Hepatitis B / metabolism
  • Hepatitis B / virology
  • Hepatitis B virus / genetics
  • Hepatitis B virus / physiology*
  • Hepatocytes / metabolism*
  • Hepatocytes / virology
  • Humans
  • Liver / metabolism
  • Liver / virology
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Tupaia* / genetics
  • Tupaia* / metabolism
  • Tupaia* / virology

Substances

  • MicroRNAs