Aerobic exercise training increases plasma Klotho levels and reduces arterial stiffness in postmenopausal women

Am J Physiol Heart Circ Physiol. 2014 Feb;306(3):H348-55. doi: 10.1152/ajpheart.00429.2013. Epub 2013 Dec 6.


The Klotho gene is a suppressor of the aging phenomena, and the secretion as well as the circulation of Klotho proteins decrease with aging. Although habitual exercise has antiaging effects (e.g., a decrease in arterial stiffness), the relationship between Klotho and habitual exercise remains unclear. In the present study, we investigated the effect of habitual exercise on Klotho, with a particular focus on arterial stiffness. First, we examined the correlation between plasma Klotho concentration and arterial stiffness (carotid artery compliance and β-stiffness index) or aerobic exercise capacity [oxygen uptake at ventilatory threshold (VT)] in 69 healthy, postmenopausal women (50-76 years old) by conducting a cross-sectional study. Second, we tested the effects of aerobic exercise training on plasma Klotho concentrations and arterial stiffness. A total of 19 healthy, postmenopausal women (50-76 years old) were divided into two groups: control group and exercise group. The exercise group completed 12 wk of moderate aerobic exercise training. In the cross-sectional study, plasma Klotho concentrations positively correlated with carotid artery compliance and VT and negatively correlated with the β-stiffness index. In the interventional study, aerobic exercise training increased plasma Klotho concentrations and carotid artery compliance and decreased the β-stiffness index. Moreover, the changes in plasma Klotho concentration and arterial stiffness were found to be correlated. These results suggest a possible role for secreted Klotho in the exercise-induced modulation of arterial stiffness.

Keywords: Klotho; aerobic exercise; arterial stiffness.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Blood Pressure
  • Case-Control Studies
  • Cross-Sectional Studies
  • Exercise*
  • Female
  • Glucuronidase / blood*
  • Humans
  • Klotho Proteins
  • Middle Aged
  • Oxygen Consumption
  • Postmenopause / blood
  • Postmenopause / physiology*
  • Vascular Stiffness*


  • Glucuronidase
  • Klotho Proteins