Real-time evaluation of aggregation using confocal imaging and image analysis tools

Analyst. 2014 Feb 7;139(3):564-8. doi: 10.1039/c3an01693e.

Abstract

Real-time confocal imaging was utilised to monitor the in situ loss of BSA monomers and aggregate formation using Spatial Intensity Distribution Analysis (SpIDA) and Raster Image Correlation Spectroscopy (RICS). At the proof of concept level this work has demonstrated the applicability of RICS and SpIDA for monitoring protein oligomerisation and larger aggregate formation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Image Processing, Computer-Assisted / methods*
  • Microscopy, Confocal / methods*
  • Protein Multimerization*
  • Protein Structure, Quaternary
  • Serum Albumin, Bovine / chemistry*
  • Time Factors

Substances

  • Serum Albumin, Bovine