[The association study of the LIPC -250g/A polymorphism and high-carbohydrate/low-fat diet induced serum lipid and apolipoprotein concentration changes in healthy youth]

Sichuan Da Xue Xue Bao Yi Xue Ban. 2013 Sep;44(5):727-30, 735.
[Article in Chinese]

Abstract

Objective: To investigate the role of the - 250G/A polymorphism in the promoter region of hepatic lipase gene (LIPC) on serum lipid profile and its interactions with a high-carbohydrate/low-fat (HC/LF) diet on serum lipid profiles in a young healthy Chinese population.

Methods: After a stabilization diet for seven days, fifty-six young healthy subjects (27 males, 29 females) were given the HC/LF diet for six days. The serum lipid profiles were analyzed of the twelve-hour fasting venous blood samples collected in the mornings of the first, the eighth and the fourteenth days. The concentrations of serum apolipoproteins were measured. The LIPC -250G/A polymorphism were analyzed.

Results: At baseline, the female subjects with the GG genotype had significantly higher high-density lipoprotein cholesterol (HDL-C) (P= 0. 041) and apolipoprotein A- I (Apo A- I ) (P= 0. 020) than the male subjects with the same genotype. After the stabilization diet, the females had significantly higher HDL-C (GG genotype: P=0. 021, A carriers: P=0. 014) and Apo A-I (GG genotype: P= 0. 035, A carriers: P= 0. 006) than the males in all genotypes. After the HC/LF diet, the female A carriers had significantly higher total cholesterol (TC) (P= 0. 042) than the male A carriers, and the females had significantly higher Apo A- I than the males in all genotypes (GG genotype: P=0. 010, A carriers: P=0. 009). Compared with thosebefore the HC / LF diet , TC ( males with GG genotype : P = 0. 013 , male A carriers: P = 0. 000 ; females with GG genotype: P=0. 025, female A carriers: P=0. 048) and low-density lipoprotein cholesterol (LDL-C) (males with GG genotype: P = 0. 028, male A carriers: P = 0. 000; females with GG genotype: P= 0. 004, female A carriers: P=0. 001) significantly decreased after the diet in all the subjects. Triglycerides (TAG) (GG genotype: P=0. 006, A carriers: P= 0. 001) significantly increased in the females regardless of the genotype. However, only in the male A carriers, HDL-C (P= 0. 011) and Apo A- I (P= 0. 041) significantly increased after the diet.

Conclusion: The A allele at the LIPC -250G/A polymorphism is associated with the HC/LF diet induced HDL-C and Apo A-I concentration changes in the males.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apolipoprotein A-I / blood*
  • Cholesterol, HDL / blood*
  • Diet, Fat-Restricted
  • Dietary Carbohydrates / administration & dosage*
  • Dietary Fats / administration & dosage*
  • Female
  • Genetic Association Studies
  • Genotype
  • Humans
  • Lipase / genetics*
  • Lipids / blood
  • Liver / metabolism
  • Male
  • Polymorphism, Genetic
  • Promoter Regions, Genetic
  • Young Adult

Substances

  • Apolipoprotein A-I
  • Cholesterol, HDL
  • Dietary Carbohydrates
  • Dietary Fats
  • LIPC protein, human
  • Lipids
  • Lipase