Selective detection of complementarity-determining regions of monoclonal antibody by limiting protease access to the substrate: nano-surface and molecular-orientation limited proteolysis

Analyst. 2014 Feb 7;139(3):576-80. doi: 10.1039/c3an02104a.

Abstract

We report a novel method for the selective proteolysis by limiting protease access to the substrate, which we have named nano-surface and molecular-orientation limited (nSMOL) proteolysis. We focus on the identification of the Fab and quantitation of antibodies. This method successfully performed limited proteolysis on the Fab.

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal / chemistry*
  • Antibodies, Monoclonal / metabolism*
  • Complementarity Determining Regions / chemistry
  • Complementarity Determining Regions / metabolism*
  • Models, Molecular
  • Molecular Sequence Data
  • Nanotechnology / methods*
  • Protein Conformation
  • Proteolysis*
  • Surface Properties
  • Trypsin / metabolism*

Substances

  • Antibodies, Monoclonal
  • Complementarity Determining Regions
  • Trypsin